rabbit anti adam 10  (Bioss)

Journal: PLoS ONE

Article Title: Central Angiotensin II Stimulation Promotes β Amyloid Production in Sprague Dawley Rats

doi: 10.1371/journal.pone.0016037

Figure Lengend Snippet: Expression analysis showing the normalized mRNA levels of ( A ) AT 1 R, ( B ) APP, ( C ) ADAM 10, BACE1 and PS1. n = 7 for AT1R and n = 6 for all others. CTs (threshold cycles) were used as the readout. The results were obtained using the comparative Ct method and the arithmetic formula 2 −ΔΔCt . Data were normalized to β-actin and represented as fold increases over saline controls. * p <0.05 or ** p <0.01 versus the control group receiving saline infusion.

Article Snippet: The following primary antibodies and working dilutions were used: rabbit polyclonal anti-APP carboxyl terminal (Sigma, 1∶5,000), rabbit polyclonal anti-ADAM 10 (Millipore, 1∶500), rabbit monoclonal anti-BACE1 (Cell Signaling, 1∶500), mouse monoclonal anti-PS1 that recognizes the PS1 amino terminal (Abcam, 1∶250), mouse monoclonal anti-AT 1 R, which recognizes the AT 1 R carboxyl terminal (Abcam, 1∶125), and mouse monoclonal anti-β actin (Sigma, 1∶1,000).

Techniques: Expressing

Journal: PLoS ONE

Article Title: Central Angiotensin II Stimulation Promotes β Amyloid Production in Sprague Dawley Rats

doi: 10.1371/journal.pone.0016037

Figure Lengend Snippet: The same gels were blotted for β-actin as an internal standard. n = 6 for each group. ( A ) Representative enhanced chemiluminescence radiographs of immunoblots showing the immunoactivities of AT 1 R, APP, ADAM 10, BACE1, PS1, C83, C99 and β-actin. Density analysis showing levels of ( B ) AT 1 R, ( C ) APP, ( D ) ADAM 10, BACE1, and PS1, ( E ) ( F ) C83 and C99. Data in ( B–E ) were normalized by dividing the density of the AT 1 R, ADAM 10, BACE1, PS1, C83 and C99 bands by the density of the β-actin band. Data in ( F ) were normalized by dividing the density of the C83 and C99 band by that of the β-actin band and then the imAPP band. All data were represented as fold increases over the saline controls. * p <0.05 or ** p <0.01 versus the control group receiving saline infusion. imAPP, immature APP; mAPP, mature APP; imADAM 10, immature ADAM 10;mADAM 10, mature ADAM 10.

Article Snippet: The following primary antibodies and working dilutions were used: rabbit polyclonal anti-APP carboxyl terminal (Sigma, 1∶5,000), rabbit polyclonal anti-ADAM 10 (Millipore, 1∶500), rabbit monoclonal anti-BACE1 (Cell Signaling, 1∶500), mouse monoclonal anti-PS1 that recognizes the PS1 amino terminal (Abcam, 1∶250), mouse monoclonal anti-AT 1 R, which recognizes the AT 1 R carboxyl terminal (Abcam, 1∶125), and mouse monoclonal anti-β actin (Sigma, 1∶1,000).

Techniques: Western Blot